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CD-Spectrometer

Introduction

CD Spectrometer measurement

Understanding protein folding...

...at very hot and very cold temperatures, in the presence of co-solvents, in induced (bio-)chemical reactions, with amino acid exchange, with spin or fluorescence labeling, in shock freezes etc.
The CD spectrometer is mainly used for the thermodynamic and kinetic characterization of protein folding. This characterization is used e.g. for the interpretation of protein-water dynamics measured by THz spectroscopy. Furthermore, this spectrometer can be used to study protein folding in the presence of co-solvents at very low and very high temperatures to understand the influence of the solvent on protein folding.


Applications and Examples

CD Spectrometer sample compartment

Possible applications:

  • circular dichroism measurements
  • linear dichroism measurements
  • various fluorescence measurements
  • induce (bio-)chemical reactions with the stopped-flow unit
  • do spectroscopy at very cold temperatures with cryostat
  • record CD melting curves and work with proteins of hyper-thermophilic organisms with Peltier-temperable cuvette holder

Set-Up

CD spectrometer stopped flow unit

The stopped-flow unit for induced reactions has to be installed separately. Please contact us.

CS/SF Stopped flow unit with sequential mixing:

  • Fully integrated stopped flow sample handling unit to enable high performance
  • fully automated stopped flow in CD, absorbance and fluorescence (with option CS/TF ) detection modes
  • 1.1ms dead time with standard 20µL volume cell
  • optical path lengths of 10mm and 2mm in absorbance mode and 5.5mm and 1.5mm in fluorescence mode , in a removable cell cartridge biocompatible micro volume sample flow circuit
  • large ratio asymmetric mixing facility (additional drives syringes for 10:1 and 5:1 mixing)
  • straightforward and rapid re-configuration
  • Xe Hg lamp in pre aligned cartridge

Specs

CD spectrometer installation cryostat
Installation cryostat
  • Advanced avalanche photodiode solid state detector with wavelength range 163nm to 1200nm (extendable to 1700nm)
  • 150W Xe arc light source
  • CD detection with simultaneous absorbance detection (i.e. not derived from detector voltage)
  • The standard detector can also be used for LD, ORD, fluorescence and fluorescence polarisation detection
  • Peltier temperature control, programmable over the range -40°C to 110°C with +/- 0.020°C precision
  • Thermal denaturation capabilities: acquisition of CD spectra (or multi-wavelength T-ramp curves) in a single continuous-T-ramp experiment.
  • External temperature probe
  • Secondary structure analysis software
  • 6 channel simultaneous data acquisition CD, (true) Absorbance, Fluorescence, HT, Voltage &Temperature

Contact

Dr. Claudius
Hoberg

Laboratory Manager -
Spectroscopy


Room: ZEMOS 0.83
Phone: +49 234 32 - 29554
E-Mail

Foto Claudius <br />Hoberg
© ZEMOS
Jens
Schulte

Technician -
Chemistry, Spectroscopy


Room: ZEMOS 0.87
Phone: +49 234 32 - 19050
E-Mail

Foto Jens <br />Schulte
© ZEMOS