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AFM - Atomic Force Microscope

Introduction

AFM

This liquid-phase AFM allows the combination of atomic force microscopy with electrochemical and optical (fluorescence-) investigations.
It provides high-resolution topographic images and simultaneously allows the localization of specific sample components (e.g. cells, biomolecules, or coat/ligand molecules on nanoparticles) via optical access for fluorescence measurements. Many questions in the field of Solvation Science are strongly dependent on electrical potentials - naturally present on the sample or externally imposed on it (surface adsorption, formation of a double layer, electrochemical reaction rates). Therefore, simultaneous imposition of electric potentials and precise measurement of the resulting currents is also possible with this AFM.


Applications and Examples

AFM joystick
  • Investigate electrocatalysis on nanoparticle-modified electrodes and magnetic field-structured electrode position using high-resolution electrochemical and magnetic atomic force microscopy (AFM, EC-AFM, MFM).
  • surface adsorption
  • formation of a double layer
  • electrochemical reaction rates
  • localize spezific sample components like cells, biomolecules, or coat/ligand molecules

Set-Up

AFM

For the different measurements (biological/electrochemical, AFM/MFM/conductive AFM, force constants, etc.) only the installation of the appropriate tip is necessary. The adjustment of the sample is easy and the alignment of the inverted fluorescence microscope compared to the lifting arm/tip is fixed thanks to the sample scanner setup with inverted microscope, so no adjustment is necessary in this respect. The respective sample holders (electrochemistry measuring cell, perfusion stages, pump, potentiostat, optical filters) are available at the AFM measuring station and can be used by all users depending on the scientific question.


BioScope Resolve 2

  • Inverted optical microscope Axio Observer
  • connector and shutter for the combination with the AFM, incl. camera and common objectives and filters for optical (fluorescence) measurements
  • Temperature-controlled flow cell for sample holding and incubation of biological samples incl. pump system
  • Conductivity AFM module for local measurement of sample conductivity
  • Electrochemical measuring cell with flexible sample dimensions and chemical resistance to high and low pH values and solvents
  • Potentiostat/Galvanostat for the application of external potentials and measurement of the current response with high resolution (incl. very low currents)

Contact

Vanessa
Scherer

Technician
Chemistry, Spectroscopy

Room: ZEMOS 1.41
Phone: +49 234 32 - 19062
E-Mail

Prof. Dr. Kristina
Tschulik

Chair of Analytical
Chemistry II

Room: ZEMOS 1.45
Phone: +49 234 32 - 29433
E-Mail

Foto Kristina <br />Tschulik
© Tschulik