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Core Facility Synthesis and Biology

You need molecular biology equipment for a certain project? - Our Core Facility Biology might help. Please contact our Lab Manager Biology.

Biology area
© ZEMOS, Jung

We have a set of basic molecular biology equipment available as well as special instruments, e.g. ÄKTA prime 25, Tecan plate reader, mammalian cell culture or Leica THUNDER Imager 3D Cell Culture equipped with Infinity Laser Scanner.

We offer support for project design and tutorials for instrument usage and data interpretation.


Microbiology & Molecular Genetics (S1)

View into a biology lab
© ZEMOS, Jung

•    Genetic engineering, design and assembly of DNA constructs (e.g. plasmids for protein expression in bacterial and mammalian cells)
•    Fusion protein creation (protein tags for antibody detection, purification, fluorescent tags, …)
•    Teaching & training of users and visitors in most important standard techniques of molecular genetics & microbiology 
•    Equipment includes PCR cyclers, UV/Vis Spectrophotometers, Gel Electrophoresis, Gel Documentation, …


Protein Biochemistry

View into cold room
© ZEMOS, Jung

•    Protein expression and purification from E. coli (mammalian cells in near future)
•    Recombinant protein purification with ÄKTA prime 25
•    Characterization of proteins, protein complexes and the interactions of proteins using biophysical techniques
•    Teaching & training of users and visitors in most important aspects of protein expression, purification and biophysical characterization techniques
•    Equipment includes UV/Vis Spectrophotometers, Thermocyclers, 2D SDS PAGE, Gel Documentation System, a Sonicator, …


Microscopy

Microscopy image
Image showing stained mitochondrial networks of HeLa cells.

•    Leica DMi8 S Fluorescence Microscope with 488 nm Laser Scanner for light patterning and photo manipulation (e.g. optogenetics, activation, bleaching, uncaging, …)
•    Confocal laser scanning microscope (Olympus IX 73) with PicoQuant MicroTime200 for time correlated single photon counting (TCSPC)
•    Localization of proteins, protein complexes and interaction studies of proteins, time lapse microscopy, fluorescence decay measurement, diffusion behaviour analysis, …


Cell Culture (S1)

View into cell culture lab
© ZEMOS, Jung

•    Mammalian cell culture BSL1 & BSL2
•    Protein expression, interaction studies, sample preparation for microscopy, …
•    Equipment includes 3 Biosafety Cabinets Class 2, ZOE Fluorescent Cell Imager, Microplate Reader, …


Contact

Dr. Sebastian
Jung

Laboratory Manager
Biology



Room: ZEMOS 1.29
Phone: +49 234 32 - 24740
E-Mail

Foto Sebastian <br />Jung
© ZEMOS
Prof. Dr. Sebastian
Kruss

Physical
Chemistry II



Room: NC 6/25
Phone: +49 234 32 - 29946
E-Mail

Foto Sebastian <br />Kruss
© RUB, Marquardt